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ผลงานตีพิมพ์ในวารสารวิชาการMulti-scale comparative transcriptome analysis reveals key genes and metabolic reprogramming processes associated with oil palm fruit abscissionผู้แต่ง:Kim Fooyontphanich, Fabienne Morcillo, Thierry Joet, Stephane Dussert, Julien Serret, Myriam Collin, Philippe Amblard, Sithichoke Tangphatsornruang, Mr.Peerapat Roongsattham, Assistant Professor, Dr.Chatchawan Jantasuriyarat, Associate Professor, Jean-Luc Verdeil, Timothy J. Tranbarger, วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการPalmelloid Formation and Cell Aggregation Are Essential Mechanisms for High Light Tolerance in a Natural Strain of Chlamydomonas reinhardtiiผู้แต่ง:Suwannachuen, N., Leetanasaksakul, K., Roytrakul, S., Phaonakrop, N., Thaisakun, S., Mr.Peerapat Roongsattham, Assistant Professor, Dr.Chatchawan Jantasuriyarat, Associate Professor, Dr.Nuttha Sanevas, Assistant Professor, Dr.Anchalee Sirikhachornkit, Associate Professor, วารสาร: |
การประชุมวิชาการLight Stress Responses – Lessons from Natural Strains of the Green Microalga Chlamydomonas Reinhardtiiผู้แต่ง:Dr.Anchalee Sirikhachornkit, Associate Professor, Nittaya Suwannachuen, Kantinan Leetanasaksakul, Sittiruk Roytrakul, Narumon Phaonakrop, Siriwan Thaisakun, Mr.Peerapat Roongsattham, Assistant Professor, Dr.Chatchawan Jantasuriyarat, Associate Professor, Dr.Nuttha Sanevas, Assistant Professor, การประชุมวิชาการ: |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Hd1, Hd3a, and Hd6 Genes : Possible DNA Methylation Roles in Photoperiod Sensitive Gene Regulation of Rice KDML 105 (Oryza sativa L.)) ผู้เขียน:พีรภัฏ รุ่งสัทธรรม, นายประดิษฐ์ พงศ์ทองคำ, ศาสตราจารย์เกียรติคุณ, นางอมรา ทองปาน, รองศาสตราจารย์, ดร.ลิลลี่ กาวีต๊ะ, รองศาสตราจารย์, ดร.ปุณฑริกา หะริณสุต, รองศาสตราจารย์, สุรินทร์ ปิยะโชคณากุล สื่อสิ่งพิมพ์:pdf AbstractThe study was aimed to investigate DNA methylation in rice after treatment with different photoperiods and to determine allelic differences of Hd1, Hd3a, and Hd6 genes, involved in photoperiod sensitivity, in KDML 105, a photoperiod-sensitive cultivar, and in Suphan Buri 1, a photoperiodinsensitive cultivar. Detection of DNA methylation was performed by Southern hybridization. The genomic DNA of KDML 105 was digested by HpaII or MspI restriction enzymes, separated by electrophoresis, Southern blotted and hybridized with probes amplified from the 5' region of Hd1, Hd3a, and Hd6 genes, respectively. The results showed no difference in band patterns of long-day treatment and short-day treatment of either HpaII or MspI digested groups indicating that the target sequences were not methylated. Primers were designed to amplify the overlapping fragments of Hd1, Hd3a, and Hd6 alleles from rice KDML 105 and Suphan Buri 1 to make a contig sequence covering the whole genes. Nucleotide and deduced amino acid sequences data obtained from this experiment indicated that Hd1, Hd3a, and Hd6 genes of KDML 105 and Suphan Buri 1 encoded normal Hd1, Hd3a, and Hd6 proteins, respectively. Taken together, the data suggested that DNA methylation and single amino acid polymorphisms (SAPs) might not be the mechanisms underlying the expression and function of photoperiod genes. However, the differences of the nucleotide in the 5' control region might be the cause of the photoperiod sensitivity difference between KDML 105 and Suphan Buri 1. |
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Researcherดร. อรินทิพย์ ธรรมชัยพิเนต, ศาสตราจารย์ที่ทำงาน:ภาควิชาพันธุศาสตร์ คณะวิทยาศาสตร์ สาขาที่สนใจ:Biology and Molecular Genetics of Streptomyces and related genera, Genetics and Biosynthetic Pathway of Polyketide Antibiotics, Combinatorial Biosynthesis, Endophytic actinomycetes: plant growth regulating agents and bioactive compounds Resume |
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